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Paucimonas lemoignei PhaZ7 depolymerase is the only extracellular depolymerase that has been described as being active towards amorphous PHAs. In order to address questions regarding the catalytic mechanism and substrate binding, the atomic resolution structure of PhaZ7 was determined after cocrystallization with the protease inhibitor PMSF. The reported structure has the highest resolution 1. Structural comparison with the free PhaZ7 structure 1. The oxyanion hole was found to be formed by the amide groups of Met and Asn Nine well ordered water molecules were located in the active site. Manual docking of a substrate trimer showed that the positions of these water molecules coincide well with the substrate atoms.

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2x5x You are using a web browser that we do not support, 2x5x. Interactions Focus chain I [auth A]. Manual docking of a substrate trimer showed that the positions of these water molecules coincide well with the substrate atoms.

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Wakadkar, S. Paucimonas lemoignei PhaZ7 depolymerase is the only extracellular depolymerase that has been described as being active towards amorphous PHAs. Paucimonas lemoignei. Macromolecule Content Total Structure Weight: This is version 1. Small Molecules. Paucimonas lemoignei. The structure of PhaZ7 at atomic 1. Rewrite using the commutative property of multiplication. Wakadkar, S. Interactions Focus chain H [auth A]. Subtract from both sides of the equation. Structural comparison with the free PhaZ7 structure 1. In order to address questions regarding the catalytic mechanism and substrate binding, the atomic resolution structure of PhaZ7 was determined after cocrystallization with the protease inhibitor PMSF.

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Asymmetric Unit. Interactions Focus chain I [auth A]. Revision History Full details and data files Version 1. Our website will not work properly. Sequence Annotations Expand. Rewrite using the commutative property of multiplication. Factor by grouping. Interactions Focus chain I [auth A]. Interactions Focus chain H [auth A]. The structure of PhaZ7 at atomic 1. Small Molecules.