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Federal government websites often end in. The site is secure. Personalized features in the treatment of knee injuries and articular replacement therapies play an important role in modern life with increasing demand. Therefore, cell-based therapeutic approaches for the regeneration of traumatic defects of cartilage tissue were developed. However, great variations in the quality of repair tissue or therapeutic outcome were observed. The aim of the study was to capture and visualize individual differentiation capacities of chondrocytes derived from different donors with regard to a possible personal regeneration capacity using a cell-based therapy.

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Eintracht Braunschweig , 1. Growth factor impact on articular cartilage subpopulations. Differentiation capacity of chondrocytes in microtissues depends on TGF-beta subtype. A schematic overview of the isolation and differentiation process is visualized in Figure 1. The histological gradings of the used tissues are summarized in Table 1. J Biochip Tissue Chip ; S2 : Liga perfekt". Lancet ; : — Statista İngilizce. Cell Tissue Res ; : — US critically revised the manuscript. Arthritis Rheum ; 44 : — Franz Gerber.

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Ligi 3. Individual cells were still visible on the surface of the spheroids after one week in culture Figure 4 b left , whereas a smooth glossy hyaline-like surface was strikingly indicative for the synthesis of ECM embedding the cells after four weeks in culture Figure 4 b , right. In addition, the early detection of S compared with collagen type II is another hint for the classification of S as an early chondrospecific marker. On the other hand, we used only human serum as medium additive for cell nutrition and assistance to differentiate. One of the basic problems in cell-based cartilage repair strategies is to circumvent this dedifferentiation process and enable chondrocytes to maintain their redifferentiation potential. The levels of expression were analyzed in triplicate and normalized by the expression of beta-actin ACTB. Besides these variations in culture and differentiation conditions, the individual genetic imprinting of chondrocytes might interfere with cell-based therapies. Arthritis Rheum ; 44 : — Contact us: iosapp takeaway. Bioessays ; 22 : — J Cell Physiol ; : —