Dehydratase

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Dehydratases are a group of lyase enzymes that form double and triple bonds in a substrate through the removal of water. Dehydratases can act on hydroxyacyl-CoA with or without cofactors , and some have a metal and non-metal cluster act as their active site. A dehydratase deficiency in the body can lead to a less severe condition of hyperphenylalaninemia , which involves an over presence of phenylalanine in the blood. It is caused by a genetic recessive disorder in the autosomal DNA. This enzyme -related article is a stub. You can help Wikipedia by expanding it. Contents move to sidebar hide.

Dehydratase

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. The members of this family are involved in various biosynthetic and carbohydrate metabolic pathways. The active site is located at the monomer-monomer interface and contains residues from both the N-terminal recognition helix and the C-terminus of the dimeric counterpart. The active site also contains a conserved Ser, which probably acts as a base in catalysis. Mariana A. Morais, Joan Coines, … Mario T. There is increasing interest in developing economical and sustainable bioprocesses to convert biomass into valuable organic compounds. Metabolic engineering and synthetic biology have enabled the development of optimized microbial strains for the production of target compounds by taking advantage of known pathways and enzymes. To fully exploit the possibilities offered by nature, a more detailed molecular-level understanding of key metabolic enzymes is required.

PksH was amplified from B. These dehydratase are mediated through activation of the VDR, which alters the transcription rates of target genes responsible for the biological response Dusso et al, dehydratase.

Federal government websites often end in. The site is secure. Lead poisoning affects many organs in the body. Our meta-analysis studied the effects of the ALAD polymorphism on a blood and bone lead levels and b indicators of target organ toxicity. Sample sizes, means, and standard deviations were extracted for the genotype groups. There was a statistically significant association between ALAD2 carriers and higher BLL in lead-exposed workers weighted mean differences of 1.

Candida albicans , a human fungal pathogen, undergoes morphogenetic changes that are associated with virulence. We report here that GAL in C. We found that GAL deletion leads to greater sensitivity to antifungal drugs and cell wall destabilizing agents like Calcofluor white and Congo red. The mutant also formed biofilms consisting mainly of hyphal cells that show less turgor. The NMR analysis of cell wall mannans of gal deletion strain revealed that a major constituent of mannan is missing and the phosphomannan component known to affect virulence is greatly reduced.

Dehydratase

Dihydroxy-acid dehydratase DHAD plays an important role in the utilization of glycerol or glucose for the production of value-added chemicals in the in vitro synthetic enzymatic biosystem. The low activity of DHAD in the dehydration of glycerate to pyruvate hampers its applications in biosystems. The shrunken substrate-binding pocket and newly formed hydrogen bonds were the reason for the activity improvement of the mutant. For the in vitro synthetic enzymatic biosystems of converting glucose or glycerol to L-lactate, the biosystems with the mutant SsDHAD showed 3.

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The key features on the monomer-monomer interface are marked with arrows. E Details of the PksH active site with key amino acids and secondary structure elements highlighted. WT PksI was recombinantly overexpressed in E. Sorry, a shareable link is not currently available for this article. Based on our available crystal structures both Lys80 and Lys were identified as potential candidates for this role in PksI. You can also search for this author in PubMed Google Scholar. Increased nitric oxide inactivation by reactive oxygen species in lead-induced hypertension. The members of this family are involved in various biosynthetic and carbohydrate metabolic pathways. Fleming et al. There is evidence that inclusion of studies that deviate from HWE can affect the pooled estimate and be potential sources of heterogeneity across the studies Trikalinos et al. Our meta-analysis did not find a significant association between ALAD polymorphism and accumulation of lead in the different bone compartments. The Mechanism of 6-Phosphogluconic Dehydrase.

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Genetic analysis of a novel pathway for D-xylose metabolism in Caulobacter crescentus. Operating characteristics of a rank correlation test for publication bias. Cite this article Rahman, M. Although both constructs encode N-terminally hexa-his tagged proteins, these tags may be cleaved using different proteases; PksH thrombin cleavable, PksI C3 cleavable. An alternative approach is the transfer of HCS-cassette enzymes and their cognate ACP domains into heterologous systems, in an effort to direct targeted branch incorporation into non-branched polyketides Applied Microbiology and Biotechnology There is evidence that inclusion of studies that deviate from HWE can affect the pooled estimate and be potential sources of heterogeneity across the studies Trikalinos et al. Published : 18 September Publish with us For authors Language editing services Submit manuscript. The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase. Am J Epidemiol. Connor, M. McCoy, A.