Luminex assay

Luminex assay is a bead-based immunoassay multiplex technique that allows multiple analytes ex. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex up to compatible luminex assay simultaneously using a commercial or custom kit, luminex assay. Over the past few luminex assay, Luminex cytokine assays have been one of our most requested biomarker testing services.

Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes.

Luminex assay

Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. This material is intended for International non-USA site visitors only. Multiplexing is a method for high-volume biomarker testing—or testing multiple analytes simultaneously within a single run—using a single sample volume. Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously. Looking for custom assay design and development services with unique targets in mind? Please select your location below Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. International US. Document repository Product documentation. Need more information? Contact us. Need any help? Ask for support. Quick links.

Typically, we utilize this format when the target molecule has a low molecular weight or when only one antibody luminex assay available.

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Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes. These unique spectral addresses are created by internally labeling beads with different ratios of two fluorophores, one in a red wavelength and the other in the far red. This is the xMAP technology. Proteins including antibodies, ligands, and nucleic acids specific to the desired targets can be coupled to the beads. Bio-Plex assays use antibodies coupled to the beads. In the kits, every antibody to a different target is covalently attached to beads with a different single spectral address, giving each target its own unique address.

Luminex assay

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Back Close Menu. One common challenge for Luminex assay multiplex tends to be finding a dilution factor that allows all analytes to be analyzed simultaneously. Partners Discover our featured Partners. NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. After data collection on the Luminex platform, we examine the calibration curves for every analyte of the multiplex immunoassay for accuracy and precision. International US. Typically, we utilize this format when the target molecule has a low molecular weight or when only one antibody is available. What is Luminex Assay? Bio-Plex Suspension Array Systems Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. We can also increase the number of bead events analyzed for each analyte if a more significant statistical analysis is required than stated in the Luminex multiplex immunoassay protocol. Looking for custom assay design and development services with unique targets in mind?

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Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. High-performance multiplex assay kits available through this platform can be a valuable asset in basic and translational research on various diseases for the simultaneous analysis of multiple proteins, including growth factors and cytokines such as interferons, interleukins, chemokines, and members of the tumor necrosis superfamily. The time it takes to evaluate the multiplex immunoassays is related to the concentration of each microsphere and the number of events recorded for each analyte. That said, the analytes of interest are sometimes too far apart in biological concentrations. Can Luminex Assays multiplex analytes that occur in vastly different biological concentrations e. That said, incubation times between steps for each Luminex based assay can vary but are tightly controlled to retain high inter-assay accuracy and precision. We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects. International US. Flexibility to singleplex or multiplex, simultaneously measuring many targets in a single sample Reduced cost and labor by multiplexing Track biological interactions in real time Luminex assays have smaller sample requirements than other techniques such as ELISA Beads have a larger surface area than a flat surface, increasing the range of detection Liquid reaction kinetics give faster, more reproducible results than planar arrays Single platform for a wide variety of applications, including analysis of proteins including phosphoproteins , signaling molecules and nucleic acids Wide variety of Luminex kits designed for disease, cytokine, growth factor, etc. For several Luminex multiplex assay or sponsor developed custom Luminex assay, validation starts with verifying appropriate reference standards, critical reagents, calibrators, and controls. Login Register.