Proc natl acad sci u s a

National Academy of Sciences.

Abstract : A key component of scientific communication is sufficient information for other researchers in the field to reproduce published findings. For computational and data-enabled research, this has often been interpreted to mean making available the raw data from which results were generated, the computer code that generated the findings, and any additional information needed such as workflows and input parameters. Many journals are revising author guidelines to include data and code availability. This work evaluates the effectiveness of journal policy that requires the data and code necessary for reproducibility be made available postpublication by the authors upon request. We assess the effectiveness of such a policy by i requesting data and code from authors and ii attempting replication of the published findings. We chose a random sample of scientific papers published in the journal Science after the implementation of their policy in February We find this policy—author remission of data and code postpublication upon request—an improvement over no policy, but currently insufficient for reproducibility.

Proc natl acad sci u s a

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. The immobilized proteins were detectable by immunological procedures. All additional binding capacity on the nitrocellulose was blocked with excess protein; then a specific antibody was bound and, finally, a second antibody directed against the first antibody. The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. In the latter case, as little as pg of protein was clearly detectable. It is anticipated that the procedure will be applicable to analysis of a wide variety of proteins with specific reactions or ligands. Abstract A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Substances Antigens Ribosomal Proteins Cellulose.

School News. Poughkeepsie, New York. Bibcode : PNAS.

Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information. MitoPedia alert. MiP and biochemistry. O2k and HRR.

As the area of small molecules interacting with RNA advances, general routes to provide bioactive compounds are needed as ligands can bind RNA avidly to sites that will not affect function. A non-oligonucleotide-containing compound was designed from sequence to target the precursor to oncogenic microRNA pre-miR for enzymatic destruction with selectivity that can exceed that for protein-targeted medicines. The compound specifically binds the target and contains a heterocycle that recruits and activates a ribonuclease to pre-miR to substoichiometrically effect its cleavage and subsequently impede metastasis of breast cancer to lung in a mouse model. Transcriptomic and proteomic analyses demonstrate that the compound is potent and selective, specifically modulating oncogenic pathways. Thus, small molecules can be designed from sequence to have all of the functional repertoire of oligonucleotides, including inducing enzymatic degradation, and to selectively and potently modulate RNA function in vivo. Keywords: RNA; cancer; chemical biology; metastatic; nucleic acids.

Proc natl acad sci u s a

Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Given the selective value of such repeats, it is likely that several different mechanisms have been responsible for their generation. One mechanism that has been shown to generate relatively long tandem repeats in the kilobase range is rolling circle replication. In this communication, we demonstrate that rolling circle synthesis in a simple enzymatic system can produce tandem repeats of monomers as short as 34 bp.

2pm est to central time

Impact factor. This page was last edited on 22 March , at Los Angeles Times. ISSN Portal. View source. MitoFit Preprints. BFI journal list. IV:5 — via Newspapers. For other uses, see PNA disambiguation. The method results in quantitative transfer of ribosomal proteins from gels containing urea. The immobilized proteins were detectable by immunological procedures. Science Citation Index Expanded. English Wikipedia.

Glucagon-like peptide-1 receptor GLP-1R agonists are efficacious antidiabetic medications that work by enhancing glucose-dependent insulin secretion and improving energy balance.

Page forms Add reference. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. School News. Page forms Add reference. HathiTrust ID. Koshland, Jr. Wikibooks 0 entries edit. Finnish Wikipedia. Read Edit View history. February July 6, The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. Printable version.