Smai cut site

Enzymes and Inhibitors. Restriction Enzymes.

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Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang.

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Smai cut site

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately enzymes. The following information is given:.

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Note: You will not be able to access your account until your email is verified. Manos UK. Preference Cookies We use these cookies to remember your settings and preferences. This product is discontinued. Account is invalid. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation This will prevent the Sma I ends of the vector from religating. If you still need the blunt end, you can just do a fill-in or chew back the overhang. Select All. You have 2 remaining attempts. United States. Microwave a mL bottle of Type I water for 1 minute on High. Search by lot number or partial lot number. We use these cookies to remember your settings and preferences. Efficiently ligates DNA inserts into plasmid vectors in just 5—15 minutes at room temperature.

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Europe Austria. This field is required. Good luck!!. Related applications: Restriction Enzyme Cloning. Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out. Not for Use in Diagnostic Procedures. Email Address Please enter a valid email address. So my situation now is I'm trying to cut a plasmid bp with smaI. After that, you will need to contact Customer Service to unlock your account. A password reset email has been sent to the primary email address associated with your account. Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? Request a Certificate.