Spectra viewer

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The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters. Download and share fluorochrome spectrum alongside laser and filter configurations Generate, export, and share the final report.

To change the current selection, click a different dye name. To select all of a range of dyes, click the first desired choice, hold the SHIFT key while clicking the dye at the end of spectra viewer desired range.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x. Add a spectrum to begin. Compound Name. How to use this tool AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats.

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Add a spectrum to begin. To exit, click "cancel". You may add further fluorophores, light sources and filters at any point, or remove them by clicking X. Contact us Company. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Products Services Tools Resources. Sign In. To view a known dye, scroll down the menu on the left and click to select. To change the current selection, click a different excitation source title. The filter will now appear on the display as a semitransparent vertical band in the color associated with its location on the spectrum. Spectra Viewer and Experiment Design. Adding Filters [ Show ] To add one or more filters, click "Filter" in the "Add" submenu on the left part of the screen. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time.

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Cart 0 Sign In. Then enter in the bandwidth number into the new input box and click "OK". If the desired excitation source is known, click to select. Adjusting Graph Display [ Show ] If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it. To keep the current number, click "Cancel", or enter the desired starting wavelength into the input box and click "OK". A - in a table cell represents no applicable spillover. AAT Bioquest. Note: if an excitation source is added to the graph, the rightmost column in the information table at the bottom of the page, labeled "Peak Intensity" with the excitation source title in parentheses will show the percentage of the maximum possible intensity for the emission curve of each compound currently on the graph. If the "Custom" row is selected, a popup box will appear and prompt the user to enter the center wavelength. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. Click "Cancel" to keep the current value or enter the desired numerical value into the input box and click "OK". To select multiple individual options at the same time, hold the CTRL key while clicking each desired dye. Use the right-hand scroll bar to view the lower section of the tables if necessary. To search for an appropriate filter, use the right-side menu section to search by part type, manufacturer, or instrument. To select all of a range of dyes, click the first desired choice, hold the SHIFT key while clicking the dye at the end of the desired range.