Sybr green master mix

Mixes are also available for fast PCR.

Explore high-quality enzymes; now available as individual product s. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies — all in GeneGlobe. High amplification efficiency over a wide dynamic range. Tighter control of polymerase activity yields greater specificity. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer—dimers and other nonspecific products.

Sybr green master mix

Yellow sample added to blue master mix yields green qPCR reaction. Visually confirm that your DNA sample was added to master mix. The hot-start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation including primer-dimers , yielding superior performance and sensitivity. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed. PCR Equipment and Supplies. Catalog number: Technical Support Customer Service. Catalog Number. Price: ,

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Mixes are also available for fast PCR. The quantity of dsDNA product in the reaction is proportional to the amount of fluorescence. Generally, probes designed for PCR are oligos that have a fluorescent molecule on one end and a quencher on the other. When the oligo primes a PCR reaction and is incorporated into a PCR product, the fluorescent molecule is no longer quenched. Most probe systems require different probes for different sequences.

Sybr green master mix

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Inspect mode — when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. Log Out. Supporting data and figures High amplification efficiency over a wide dynamic range. Yellow sample added to blue master mix yields green qPCR reaction. This Mastermix should be used in qPCR instruments under standard cycling conditions. Fluorescence intensity is monitored during this final temperature increase, resulting in the generation of a melting curve or dissociation curve. There are several reasons for not seeing a PCR product. Login Register. Contains 24 x 1. Try the Workflow Configurator. Price: ,00 Online price: ,65 Your price: Sign In. Contains 6 x 1. Back Close Menu. HotStart enzymes are inactive at room temperature, and require heating at nucleic acid melting temperatures in order to be activated.

Explore high-quality enzymes; now available as individual product s. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies — all in GeneGlobe.

Price: 2. Fluorescence intensity is monitored during this final temperature increase, resulting in the generation of a melting curve or dissociation curve. Specialties Back Close Menu. PCR Equipment and Supplies. Yellow sample added to blue master mix yields green qPCR reaction. Contains 24 x 1. For pathway-focused gene expression analysis. Request a Certificate. You can create and edit multiple shopping carts. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies — all in GeneGlobe.